Rubiscolin: Natural Selective Delta Agonists

[Paregoric Kid]

anyone have any additional info?

The rubiscolins are a group of opioid peptides (http://en.wikipedia.org/wiki/Opioid_peptide) which are formed during digestion of the ribulose bisphosphate carboxylase/oxygenase (Rubisco (http://en.wikipedia.org/wiki/Rubisco)) protein from spinach (http://en.wikipedia.org/wiki/Spinach) leaves. Rubiscolins mimick the effects of opiates and therefore influence the mind. These peptides have much in common with the more known gluten exorphins (http://en.wikipedia.org/wiki/Gluten_exorphin). The rubiscolins' impact on mental health (http://en.wikipedia.org/wiki/Mental_health) is not yet known.
There are 2 known rubiscolins with known structure:
Rubiscolin-5


Structure: H-Gly (http://en.wikipedia.org/wiki/Glycine)-Tyr (http://en.wikipedia.org/wiki/Tyrosine)-Tyr-Pro (http://en.wikipedia.org/wiki/Proline)-OH
Chemical formula: C30H45N5O9
Molecular weight: 619.70 g/mol Rubiscolin-6


Structure: H-Gly-Tyr-Tyr-Pro-Thr (http://en.wikipedia.org/wiki/Threonine)-OH
Chemical formula: C39H54N6O10
Molecular weight: 766.87 g/mol
Effect of rubiscolin, a delta opioid peptide
derived from Rubisco, on memory consolidation
by
Yang S, Kawamura Y, Yoshikawa M.
Division of Food Bioscience and Biotechnology,
Graduate School of Agriculture,
Kyoto University, Uji,
611-0011, Kyoto, Japan.
Peptides. 2003 Feb;24(2):325-8

ABSTRACT

Rubiscolin-6 (YPLDLF) is a delta selective opioid peptide isolated from the enzymatic digests of ribulose bisphosphate carboxylase/oxygenase (Rubisco) from spinach leaves. In a step-through type passive avoidance test in ddY mice, rubiscolin-6 enhanced memory consolidation at doses of 3nmol/mouse after intracerebroventricular administration, and at 100mg/kg after oral administration. These doses are smaller than the optimal doses for an analgesic effect. The memory enhancing effect of rubiscolin-6 was blocked by pretreatment with the delta antagonist naltrindole, suggesting the involvement of the delta opioid receptor.

[Hammilton]

I did some looking and they seem really interesting. I doubt they'll have any sort of abuse potential, I think they'd be the first peptide to do so. Proteins, man. lol


Anyway, they probably have their place in the prevention of tolerance

intracerebroventricular adminstration- that's gotta be the SHIT!! Can you imagine how little H you'd need to get off with this method?

3nmol by intra-CBV adminstration to 100mg/kg oral? That's fucking nuts.

[Paregoric Kid]

first abusable peptide? not in a long shot, look up dermorphin

[Hammilton]

Yeah, I guess if you want to end up autistic, dermorphin might be abusable...

although there's absolutely no evidence to indicate that it'd be euphoric in humans. Looking at all the research, this is probably the opiopeptide least likely to be abusable in humans for the simple fact that it's a potential cause of some serious psychiatric problems

[Hammilton]

Oops, I do that back my comment though. There's a peptide that actually has abuse potential and makes me want to "turn on"

Bremelanotide



that's one worth checking out. I should start a thread on it in OD

[Paregoric Kid]

dermorphin already has been abused! it is euphoric, there are people who have done it and liked it and years later they're still fine, no autism. it just costs too much for it to be widely abused. just because it is found in autistic people doesn't necessarily mean it causes autism, especially in people that don't have autism already or don't have it in their family history. I have some theories regarding opiates and autism, I'll try and post it sometime. please don't be quick to jump to the conclusion that OMG IT CAUSES AUTISM just from reading about it being in autistic people. it is sometimes found in people who don't even have autism! there is a long history of it's use with some of the natives in south america, who use it by taking the skin secretions from the frog Phyllomedusa sauvaei (which contains other peptides too). believe me dermorphin is pretty damn good.

[Papa Verine]

I'm eating a big-ass bowl of spinach leaves for breakfast, lunch and dinner today.

[Hammilton]

yeah, you're right pg. that's an over simplification. I think that should certainly give pause before anyone goes from use, to abuse. There's a big difference in once-in-a-while and i'm-neve-sober usage, especially in terms of risks. Based on the data I've seen, I definitely wouldn't want to be the first person to use it.

and the serious psychiatric harm I was referring to wasn't autism. There seems to be a connection between serious psychiatric conditions and it's presence in the body.

[Opilover]

Ok does this mean that spinach might be helpful in WDs? or am I reading this topic wrong?

[Paregoric Kid]

could you show me this data? but like I said its presence in the body of people with ANY condition doesn't necessarily mean that it causes the condition. there are some people here at opiophile and some over at bluelight that have tried this out. it was a really interesting peptide but as soon as more people started to use it I'm pretty sure most of the suppliers started raising the price of it (not sure but if true the raising of its price could just be a supply/demand problem or the suppliers catching on). if I was rich I'd buy a big supply of dermorphin.

[jacky]

people have used dermorphin for possibly hundreds of years, and the western researchers that bioassayed the frog venom source for dermorphin reported lasting beneficial side effects after the initial barrage of side effects caused by the other poisons in the frog venom were dealt with....

south american natives use the frog venom for hunting....even giving the stuff to their dogs to increase awareness

but if my memory serves right, dermorphin in its pure form is a very short acting compound.

most, if not all the peptides that have potential for abuse are still up for sale on different peptide companies websites, there are even a few designed to resisnt enzyme catabolism that can extend the effects of said peptide from a few minutes, to a few hours...

my personal interest is casien/casomorphin peptides, and of course this rubiscolin.....I have consumed them all my life in the natural states, and I dont really have a worry about someday trying them in their pure form, save for being very careful, and properly starting in the low microgram amount to begin the tests....

remember, opiates, endogenous ones especially, can have very potent and beneficial effects when it comes to awareness, novel information aquisition, motivational drive....without these endogenous peptides that have opiate activity, there would be no focus, no arrangement and recall of information...
they are just as important as dopamine in this sense....

reading J. Otts opinions on the potential of opioids being used as a smart drug got me interested in this realm of psychoactive discovery...

[Hammilton]

Okay- I must be absolutely retarded because I can't find anything about this. I'm obviously entirely confused on the subject or going about it in the wrong way.I was obviously wrong