jacky
04-14-2009, 11:03 AM
"Anti-Nociceptive Effects of an Ethanolic Extract of the Whole Plant of Synedrella nodiflora (L.) Gaertn in Mice: Involvement of Adenosinergic Mechanisms
E. Woode, P. Amoateng, C. Ansah and M. Duwiejua
Abstract: This study presents the effect of an ethanolic extract of the whole plant of Synedrella nodiflora, a plant used in Ghana for the treatment of epilepsy and pain, in formalin-induced pain and acetic acid-induced writhing assay and the possible mode(s) of action of its analgesic action. For comparison, morphine and diclofenac were used as standard opioid and NSAID respectively. The ethanolic extract (100-1000 mg kg-1; p.o.) and morphine (1-10 mg kg-1; i.p.) dose-dependently decreased both phases of the formalin-induced nociceptive behavior. The antinociceptive effect of S. nodiflora (300 mg kg-1 p.o.) on the first and second phases of formalin induced pain was significantly blocked by caffeine but not by naloxone. In the acetic acid- induced writhing test, diclofenac and S. nodiflora significantly reduced the number of writhes dose dependently. Also, the effect of S. nodiflora (300 mg kg-1 p.o.) was blocked by caffeine (3 mg kg-1 i.p.) but the analgesic effect of diclofenac was enhanced significantly. The observed effects of caffeine on the central and peripheral analgesic effects of S. nodiflora in the formalin and acetic acid induced writhing suggest the possible involvement of adenosinergic mechanism(s)."
caffeine and theophylline purine compounds very similiar to one another, are adenosine receptor antagonists.
that would explain probably, why naloxone has no effect on the reversal of pain augmentation with the above mentioned species of plant, because direct opioid activation is not present.
years ago I was told that coffee would help lengthen an opiate buzz.
then I heard that coffee contains opioid antagonist lactone compounds
heres a clip
"We have reported previously that instant coffee contains ligands for opiate receptors with characteristics similar to those of opiate antagonists.
2. A concentrate of receptor-active ligands from instant coffee was prepared by serial treatments involving Amberlite XAD-2, flash chromatography and gel permeation chromatography.
3. Examination of the final concentrate by GC-MS showed the presence of a number of isomeric (iso)feruloylquinic acid lactones.
4. It is suggested that the synthesis and biological testing of each quinide isomer will establish which is responsible for the opiate receptor activity of instant coffee."
and another
"4-Caffeoyl-1,5-quinide in roasted coffee inhibits [3H]naloxone binding and reverses anti-nociceptive effects of morphine in mice"
which makes me think, that the reason I cannot tolerate coffee, is not because of the acid content...but because the antagonists fuck with my bowels. regardless of if I am using opiates/opioids or not.
heres another clip that deepens/broadens the subject
"Dicinnamoylquinides in roasted coffee inhibit the human adenosine transporter "
finally, I found a good article, complete with chemical structures..
under the title
"cerebral effects of non caffeine constituents in roasted coffee"
this article ponders that coffee can help withdrawl or cravings to opiates.
I wonder if micro doses of coffee, roasted or instant, would have a similiar effect to that of naloxone/naltrexone having a potentiating effect on opiates?
for some reason, tea doesnt seem to have the same effect.
so maybe the other compounds besides caffeine and theophylline are responsible for the majority of "anti" opiate effects?
I had to quit drinking coffee years ago for the most part. occasionally I can eat it in food and mixed drinks.
I avoid instant coffee, unless I am locked up and hating life. that hasnt happened for over 10 years though...so its been awhile.
E. Woode, P. Amoateng, C. Ansah and M. Duwiejua
Abstract: This study presents the effect of an ethanolic extract of the whole plant of Synedrella nodiflora, a plant used in Ghana for the treatment of epilepsy and pain, in formalin-induced pain and acetic acid-induced writhing assay and the possible mode(s) of action of its analgesic action. For comparison, morphine and diclofenac were used as standard opioid and NSAID respectively. The ethanolic extract (100-1000 mg kg-1; p.o.) and morphine (1-10 mg kg-1; i.p.) dose-dependently decreased both phases of the formalin-induced nociceptive behavior. The antinociceptive effect of S. nodiflora (300 mg kg-1 p.o.) on the first and second phases of formalin induced pain was significantly blocked by caffeine but not by naloxone. In the acetic acid- induced writhing test, diclofenac and S. nodiflora significantly reduced the number of writhes dose dependently. Also, the effect of S. nodiflora (300 mg kg-1 p.o.) was blocked by caffeine (3 mg kg-1 i.p.) but the analgesic effect of diclofenac was enhanced significantly. The observed effects of caffeine on the central and peripheral analgesic effects of S. nodiflora in the formalin and acetic acid induced writhing suggest the possible involvement of adenosinergic mechanism(s)."
caffeine and theophylline purine compounds very similiar to one another, are adenosine receptor antagonists.
that would explain probably, why naloxone has no effect on the reversal of pain augmentation with the above mentioned species of plant, because direct opioid activation is not present.
years ago I was told that coffee would help lengthen an opiate buzz.
then I heard that coffee contains opioid antagonist lactone compounds
heres a clip
"We have reported previously that instant coffee contains ligands for opiate receptors with characteristics similar to those of opiate antagonists.
2. A concentrate of receptor-active ligands from instant coffee was prepared by serial treatments involving Amberlite XAD-2, flash chromatography and gel permeation chromatography.
3. Examination of the final concentrate by GC-MS showed the presence of a number of isomeric (iso)feruloylquinic acid lactones.
4. It is suggested that the synthesis and biological testing of each quinide isomer will establish which is responsible for the opiate receptor activity of instant coffee."
and another
"4-Caffeoyl-1,5-quinide in roasted coffee inhibits [3H]naloxone binding and reverses anti-nociceptive effects of morphine in mice"
which makes me think, that the reason I cannot tolerate coffee, is not because of the acid content...but because the antagonists fuck with my bowels. regardless of if I am using opiates/opioids or not.
heres another clip that deepens/broadens the subject
"Dicinnamoylquinides in roasted coffee inhibit the human adenosine transporter "
finally, I found a good article, complete with chemical structures..
under the title
"cerebral effects of non caffeine constituents in roasted coffee"
this article ponders that coffee can help withdrawl or cravings to opiates.
I wonder if micro doses of coffee, roasted or instant, would have a similiar effect to that of naloxone/naltrexone having a potentiating effect on opiates?
for some reason, tea doesnt seem to have the same effect.
so maybe the other compounds besides caffeine and theophylline are responsible for the majority of "anti" opiate effects?
I had to quit drinking coffee years ago for the most part. occasionally I can eat it in food and mixed drinks.
I avoid instant coffee, unless I am locked up and hating life. that hasnt happened for over 10 years though...so its been awhile.